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Instruction Manual
Instruction Manual
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Mi455 Mini-titrator for the determination of FREE & TOTAL SULPHUR DIOXIDE in wine analysis

Mi455 is a user-friendly microprocessor-based mini-titrator for the determination of free and total sulphur dioxide in the process of wine making. This mini-titrator will give you direct readings with a range of 0 to 400 ppm.
The instrument comes with a pre-programmed analysis method for free and total sulphur dioxide measurements on wine sample.

Technically, sulphite is a salt or ester of sulfurous acid, but more commonly, sulfur dioxide (SO2). Sulfite is the most effective and widely used preservative in winemaking. It preserves by safeguarding musts and wines against premature oxidation and microscopic life forms that could otherwise spoil wine. It preserves a wine’s freshness, helps maintain its color, and is essential for aging wines beyond their first year without deterioration. It also inhibits wild yeasts, thereby allowing cultured wine yeasts to dominate the fermentation. Sulfites may be "bound" or "free." Bound SO2 combines with aldehyde compounds, those most responsible for oxidation in wines.

Free SO2 results from the dissipation of active SO2 and is the only SO2 that provides antiseptic and oxidative protection to wines. The most efficient what to add free SO2 to a must, juice or wine is by adding dissolved potassium metabisulfite to it. The effectiveness of free SO2 is dependent on the pH of the media to which it is added.

Mi455
Solutions and Reagents included
Calibration standard SO2, Titrant SO2, Alkaline reagent for total SO2, Acid reagent for total SO2, Acid reagent for free SO2, Stabilizer SO2, Refilling Electolyte Solution 3.5M KCl for ORP electrodes.
Easy Steps
Prepare the sample to be measured with wine and reagent, push the start button and note the reading.
SPECIFICATIONS
Range 0 to 400 ppm of SO2
Resolution 1 ppm
Accuracy 5% of reading
Method ripper titrimetric method
Principle equivelance point redox titration
Sample Volume 50 mL
ORP electrode MA924B/1 (included)
Pump debit 0.5 mL/min
Stirring Speed 1500 rpm
Environment 0 to 50°C; max RH 95%
Power Supply 220V/50 Hz; 10VA
Dimensions 208 x 214 x 163 mm
Weight 2.2 Kg

 

ACCESSORIES
Mi555-001 Calibration standard, SO2 (500 mL bottle)
Mi555-002 Titrant SO2 (100 mL bottle)
Mi555-003 Alkaline reagent for total SO2, (4 x 100 mL bottle)
Mi555-004 Acid reagent for total SO2, (4 x 100 mL bottle)
Mi555-005 Acid reagent for free SO2, (4 x 100 mL bottle)
Mi555-006 Stabilizer, SO2, (40 pp)
MA924B/1 ORP Electrode
MA9011 Refilling Electrolyte Solution 3.5M KCl,
for ORP electrodes, 230 mL bottle
ORDERING INFORMATION
Mi455 is supplied complete with:
Calibration standard SO2, Titrant SO2, Alkaline reagent for total SO2, Acid reagent for total SO2, Acid reagent for free SO2, Stabilizer, SO2, MA924B/1 ORP electrode, small stir bar, 2 x 50 mL beakers, 2 x 25 mL beakers, Refilling Electolyte Solution 3.5M KCl for ORP electrodes 230 mL bottle, test tube set, O-ring, 1 mL syringe, power cable and instruction manual.

 

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<td colspan="3" valign="top" bgcolor="#FFFFFF"><p align="justify"><font color="#003399" size="2" face="Arial, Helvetica, sans-serif"><strong>Mi456
Mini-titrator for the determination of TITRATABLE TOTAL ACIDITY for wine analysis</strong></font></p>
<p align="justify"><font size="2" face="Arial, Helvetica, sans-serif">        Mi456 is a user-friendly microprocessor-based mini-titrator for the determination of the titratable total acidity in the process of wine making. This minititrator gives you direct readings in g/L of tartaric acid, with a range of 0.0 to 25.0 g/L.</font></p>
<p align="justify"><font size="2" face="Arial, Helvetica, sans-serif">The instrument comes with a pre-programmed analysis method for the titratable total acidity measurements on wine sample. Also called TA and sometimes total acidity, titratable acidity is the sum of the fixed and volatile acids present in a wine. This is determined by a chemical process called titration. The titratable acidity is usually expressed in terms of tartaric acid, even though the other acids are also measured. Titratable acidity is expressed either as a percentage or as grams per liter. For example, 0.7% TA is the same as 7 grams per liter (or 7 g/l) TA.</font></p>
<p align="justify"><font size="2" face="Arial, Helvetica, sans-serif">Acidity in the must will result in a poor fermentation and a slightly medicinal and flat taste. Too much acid will give the wine an unpleasant sourness or tartness. Some acid is necessary for fermentation, and up to one-fourth of the initial acid content will be consumed by the yeast during fermentation. Low-acid musts are usually corrected by adding tartaric acid (the principle acid in grapes), malic acid, citric acid, or acid blend. An acid testing kit is indispensable in measuring initial acidity.</font></p></td>
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<td valign="top"><div align="justify"><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Solutions and Reagents included<br>
</font><font size="2" face="Arial, Helvetica, sans-serif">Calibration standard TA, Titrant TA, Buffer pH 7.0, Buffer pH 8.2, MA9011 Refilling Electolyte Solution 3.5M KCl, for pH electrodes</font></div></td>
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<td valign="top"><div align="justify"><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Glass electrode &amp; Temp Probe<br>
</font><font size="2" face="Arial, Helvetica, sans-serif">pH electrodes and Temperature Probe are supplied with the Mi456</font></div></td>
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<td colspan="2" bgcolor="#003399"><font color="#FFFFFF" face="Arial, Helvetica, sans-serif"><strong>SPECIFICATIONS</strong></font></td>
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<td width="37%"><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Range</font></strong></td>
<td width="63%"><font size="2" face="Arial, Helvetica, sans-serif">0.0 to 25.0 g/L of tartaric acid</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Resolution</font></strong></td>
<td width="63%"><font size="2" face="Arial, Helvetica, sans-serif"> 0.1 g/L</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Accuracy</font></strong></td>
<td width="63%"><font size="2" face="Arial, Helvetica, sans-serif"> 5% of reading</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Method</font></strong></td>
<td width="63%"><font size="2" face="Arial, Helvetica, sans-serif">
acid-base titration method</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Principle</font></strong></td>
<td width="63%"><font size="2" face="Arial, Helvetica, sans-serif">
end-point titration</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">pH calibration</font></strong></td>
<td width="63%"><font size="2" face="Arial, Helvetica, sans-serif">
1 point in selected end-point: 7.00 pH or 8.20 pH</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Sample Volume</font></strong></td>
<td width="63%"><font size="2" face="Arial, Helvetica, sans-serif">
2 mL</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Temperature Compensation </font></strong></td>
<td width="63%"><font size="2" face="Arial, Helvetica, sans-serif">
Automatic from 0.0 to 100.0&deg;C</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">pH electrode </font></strong></td>
<td width="63%"><font size="2" face="Arial, Helvetica, sans-serif">MA919B/1 (included)</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Temperature Probe</font></strong></td>
<td width="63%"><font size="2" face="Arial, Helvetica, sans-serif"> MA831R (included)</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Pump debit </font></strong></td>
<td width="63%"><font size="2" face="Arial, Helvetica, sans-serif">
0.5 mL/min</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Stirring Speed</font></strong></td>
<td width="63%"><font size="2" face="Arial, Helvetica, sans-serif"> 1500 rpm</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Environment</font></strong></td>
<td><font size="2" face="Arial, Helvetica, sans-serif"> 0 to 50&deg;C; max RH 95%</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Power Supply </font></strong></td>
<td><font size="2" face="Arial, Helvetica, sans-serif"> 220V/50 Hz; 10VA</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Dimensions</font></strong></td>
<td><font size="2" face="Arial, Helvetica, sans-serif">208 x 214 x 163
mm</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Weight
</font></strong></td>
<td width="63%"><font size="2" face="Arial, Helvetica, sans-serif">2.2
Kg</font></td>
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<td colspan="2"><font color="#FFFFFF" face="Arial, Helvetica, sans-serif"><strong>ACCESSORIES</strong></font></td>
</tr>
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<td width="20%"><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Mi556-001
</font></strong></td>
<td width="80%"><font size="2" face="Arial, Helvetica, sans-serif">Calibration standard TA (100 mL bottle)</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Mi556-002
</font></strong></td>
<td><font size="2" face="Arial, Helvetica, sans-serif">Titrant TA (100 mL bottle)</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Mi556-003
</font></strong></td>
<td><font size="2" face="Arial, Helvetica, sans-serif">Buffer pH 7.0 (100 mL bottle)</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Mi556-004
</font></strong></td>
<td><font size="2" face="Arial, Helvetica, sans-serif">Buffer pH 8.2 (100 mL bottle)</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">MA919B/1
</font></strong></td>
<td><font size="2" face="Arial, Helvetica, sans-serif">pH Electrode</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">MA831R
</font></strong></td>
<td><font size="2" face="Arial, Helvetica, sans-serif">Temperature probe</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">MA9011
</font></strong></td>
<td><font size="2" face="Arial, Helvetica, sans-serif">Refilling Electolyte Solution 3.5M KCl,<br>
for ORP electrodes, 230 mL bottle</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Mi0009
</font></strong></td>
<td><font size="2" face="Arial, Helvetica, sans-serif"> Small stir bars (5 pcs)</font></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Mi0020</font></strong></td>
<td><p><font size="2" face="Arial, Helvetica, sans-serif"> 50 mL beaker (4 pcs)<br>
</font></p></td>
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<td><strong><font color="#003399" size="2" face="Arial, Helvetica, sans-serif">Mi0022</font></strong></td>
<td><font size="2" face="Arial, Helvetica, sans-serif"> 2000 &micro;L pipette (1 pc)</font></td>
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<td><strong><font size="2"><font color="#003399" face="Arial, Helvetica, sans-serif">Mi0023</font></font></strong></td>
<td><font size="2" face="Arial, Helvetica, sans-serif"> Pipette tips for 2000 &micro;L pipette (4 pcs)</font></td>
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<td colspan="2"><font color="#FFFFFF" face="Arial, Helvetica, sans-serif"><strong>ORDERING INFORMATION</strong></font></td>
</tr>
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<td height="36" colspan="2"><div align="justify"><font size="2" face="Arial, Helvetica, sans-serif">Mi456 is supplied complete with:<br>
Calibration standard TA, Titrant TA, Buffer pH 7.0, Buffer pH 8.2, MA919B/1 pH Electrode, MA831R Temperature probe, MA9011 Refilling Electolyte Solution 3.5M KCl, for pH electrodes 230 mL bottle, small stir bar, 2 x 50 mL beakers, 2000 &micro;L pipette, test tube set, O-ring, 1 mL syringe, power cable and instruction manual.</font></div></td>
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